BIODESULFURISASI MINYAK SOLAR PRODUK KILANG PPSDM MIGAS CEPU MENGGUNAKAN BAKTERI Rhodococcus erythropolis

Alchibalt Bima Putra Dzufakar

doi:10.37525/sp/2023-1/374

Authors

Alchibalt Bima Putra Dzufakar Semarang

DOI:

https://doi.org/10.37525/sp/2023-1/374

Keywords:

Diesel oil, Biodesulfurization, Rhodococcus erythropolis

Abstract

Diesel oil is a type of fuel for diesel engines as a result of processing from crude oil. One of the content of diesel oil that causes various problems is sulfur compounds. Excess sulfur content will cause odors in processed products, the calorific value produced can decrease, causing corrosive properties in processing equipment at the PPSDM Migas refinery such as fractionation columns, refinery storage tanks, distribution pipes, pumps, and vehicle storage tanks. The limit of sulfur content in diesel oil set by the Director General of Oil and Gas is 50 ppm. This study aims to determine the ability of Rhodococcus erythropolis bacteria to biodesulfurize diesel oil, to determine the growth activity of Rhodococcus erythropolis bacteria to biodesulfurize diesel oil, and to determine whether the biodesulfurization process can cause corrosivity in diesel oil. The method used is biodesulfurization of growing cells using Rhodococcus erythropolis bacteria, sulfur testing is done by ASTM D2622 method, and corrosivity testing is done by ASTM D130 method. The treatments used in this study were variations in the pH of the growth medium, variations in the concentration of diesel oil, and variations in the length of incubation time. In this study, the bacteria Rhodococcus erythropolis succeeded in reducing the highest sulfur content in diesel oil from 2167.9 to 1766.1 (ppm) with pH 8 treatment. The biodesulfurization process caused a decrease in the pH of the media from 8.2 to 7.1 with pH treatment. 8.

References

Adnyana, G. A. B. S, Gunam, I. B. W., & Anggraeni, A. A. M. D. (2016). Penentuan suhu dan sumber karbon terbaik pada pertumbuhan isolat SBJ8 dalam biodesulfurisasi dibonzotiofena. Jurnal Rekayasa dan Manajemen Agroindustri. 4(4): 43-48.

Aggarwal, S., Karimi, I. A., Kilbane II, J. J., & Lee, D. Y. (2012). Roles of sulfite oxidoreductase and sulfite reductase in improving desulfurization by Rhodococcus erythropolis. Molecular BioSystems. 8(10): 2724-2732.

ASTM International. (2016). ASTM D2622. Standard Test Method for Sulfur in Petroleum Products by Wavelength Dispersive X-ray Fluorescence Spectrometry. United States : ASTM : International.

ASTM International. (2019). ASTM D130. Standard Test Method for Corrosiveness to Copper from Petroleum Products by Copper Strip Test. United States : ASTM : International.

Banerjee, S., Joshi, S. R., Mandal, T., & Halder, G. (2017). Insight into Cr 6+ reduction efficiency of Rhodococcus erythropolis isolated from coalmine waste water. Chemosphere. 167(2017): 269–281.

Chen, S., Zang, M., Li, L., Chen, J., Liu, Q., Feng, X., Sun, S., Zang, C., & Zhao, C. (2021). Efficient biodesulfurization of diesel oil by Gordonia sp. SC-10 with highly hydrophobic cell surfaces. Biochemical Engineering Journal. 174 (2021).

Derikvand, P., Etemadifar, Z., & Biria, D. (2015). RSM optimization of dibenzothiophene biodesulfurization by newly isolated strain of Rhodococcus erythropolis PD1 in aqueous and biphasic systems. Microbiology. 84(1): 65–72.

Guerrero, R. d. B. S., Diniz dos Santos, C. E., Soares, L. A., & Zaiat, M. (2020). Comparison between two different fixed-bed reactor configurations for nitrogen removal coupled to biogas biodesulfurization. Biochemical Engineering Journal. 162(2020): 107716

Hu, T., Yang, Y., Zhang, M., Gao, Y., Cheng, Q., & Ji, H. (2019). Biodesulfurization of coal using Rhodococcus erythropolis SX-12 and Acidithiobacillus ferrooxidans GF: A two-step approach. Energy Science & Engineering. 7(1): 162–169.

Ibrahim, M. H., Hayyan, M., Hashim, M. A., & Hayyan, A. (2017). The role of ionic liquids in desulfurization of fuels: A review. Renewable and Sustainable Energy Reviews. 76(2017): 1534–1549.

Khiar, M. S. A., Brown, R. C. D., & Lewin, P. L. (2019). Sacrificial copper strip sensors for sulfur corrosion detection in transformer oils. Measurement, 148(2019): 1-6.

Kilbane, J. J. (1990). Sulfur-specific microbial metabolism of organic compounds. Resources. Conservation and Recycling, 3(2-3): 69–79

Kilbane, J. J. II, and Stark, B. (2016). Biodesulfurization: a model system for microbial physiology research. World. J. Microbiol. Biotechnol. 32(2016):137.

Nassar, H. N., Ali, H. R., & El-Gendy, N. S. (2021). Waste prosperity: Mandarin (Citrus reticulata) peels inspired SPION for enhancing diesel oil biodesulfurization efficiency by Rhodococcus erythropolis HN2. Fuel. 294(2021): 1-16.

Ohshiro, T., Kojima, T., Torii, K., Kawasoe, H., & Izumi, Y. (1999). Purification and characterization of dibenzothiophene (DBT) sulfone monooxygenase, an enzyme involved in DBT desulfurization, from Rhodococcus erythropolis D-1. Journal of Bioscience and Bioengineering. 88(6): 610–616.

Olmo, C. H. del, Santos, V. E., Alcon, A., & Garcia-Ochoa, F. (2005). Production of a Rhodococcus erythropolis IGTS8 biocatalyst for DBT biodesulfurization: influence of operational conditions. Biochemical Engineering Journal. 22(3): 229–237.

Pikoli, M. R., Astuti, P., Ahmad, F., & Solihat, N. A. (2013). Pengayaan- bertingkat dibenzothiophene pada sampel tanah pertambangan batubara untuk mengisolasi bakteri desulfurisasi. Prosiding Semirata FMIPA Universitas Lampung. 103–110

Thompson, D., Cognat, V., Goodfellow, M., Koechler, S., Heintz, D., Carapito, C., Dorsselaer, A. V., Mahmoud, H., Sangal, V., Ismail, W. (2020). Phylogenomic classification and biosynthetic potential of the fossil fuel-biodesulfurizing Rhodococcus strain IGTS8. Frontiers in Microbiology. 11.

Yang, E., Fan, L., Yan, J., Jiang, Y., Doucette, C., Fillmore, S., & Walker, B. (2018). Influence of culture media, pH and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria. AMB Express. 8(1): 1-14.

Yu, M., Zhang, N., Fan, L., Zhang, C., He, X., Zheng, M., & Li, Z. (2015). Removal of organic sulfur compounds from diesel by adsorption on carbon materials. Reviews in Chemical Engineering. 31(1): 27-43.